LOCUS       sequence-59282-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Lentini R, Forlin M, Martini L, Del Bianco C, Spencer AC, Torino D, 
            Mansy SS
  TITLE     Fluorescent Proteins and in Vitro Genetic Organization for Cell-Free
            Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2013 Mar 15.
  PUBMED    23654270
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Mar 15."
COMMENT     Sequence Label: pET21b-RL015A
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..35)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             760..782
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttagcagccg gatctcagtg gtggtggtgg tggtgctcga gttatttata cagctcatcc
       61 ataccaccgg tagaatgacg tccttcagca cgctcatact gctccacgat cgtatagtcc
      121 tcattgtgtg aggtgatgtc cagtttgatg ttaacgttat aggcaccagg cagttggaca
      181 ggttttttgg ctttatacgt ggttttcact tcggcatcat agtggccacc atctttcagt
      241 ttcagacgct gtttgatttc gcctttcaga gcgccatctt ctggatacat acgctcacta
      301 gaggcctccc aacccatcgt ttttttctgc ataaccgggc catcactcgg aaaattcgtg
      361 ccacgcagtt tcactttata gatgaactca ccgtcttgca ggctagaatc ctgtgtcact
      421 gtcacaacac caccatcctc aaagttcatc acacgttccc atttgaaccc ttcagggaag
      481 ctcagtttca gatagtccgg aatatcggcc ggatgtttca cataggcttt ggagccatac
      541 atgaactgtg ggctcagaat atcccaagca aatggcagtg gaccaccttt cgtgactttc
      601 agtttagcgg tctgtgtacc ctcatacgga cgaccttcgc cttcgccctc aatttcgaac
      661 tcgtgaccat taacgctgcc ttccatgtgg actttgaagc gcatgaactc tttgatgatc
      721 gccatattgt cctcctcgcc tttactcacc atatgtatat ctccttctta aagttaaaca
      781 aaattattcg gatccgctta tttgtacagt tcatccatac catgcgt
//
