LOCUS       sequence-59284-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GMPR2A
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..104
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcacagc agcggcctgg ttccgcgtgg tagtctggac ttcaaggatg
      121 tccttttgag gcccaaacgc agtaccctta agtctcgaag tgaggtggat ctcacaagat
      181 ccttttcatt tcggaactca aagcagacat actctggggt tcccatcatt gctgccaata
      241 tggatactgt gggcaccttt gagatggcca aggttctctg taagttctct ctcttcactg
      301 ctgtccataa gcactatagc ctcgttcagt ggcaagagtt tgctggccag aatcctgact
      361 gtcttgagca tctggctgcc agctcaggca caggctcttc tgactttgag cagctggaac
      421 agatcctgga agctattccc caggtgaagt atatatgcct ggatgtggca aatggctact
      481 ctgaacactt tgttgaattt gtaaaagatg tacggaagcg cttcccccag cacaccatca
      541 tggcagggaa tgtggtaaca ggagagatgg tagaagagct catcctttct ggggctgaca
      601 tcatcaaagt gggaattggg ccaggctctg tgtgtactac tcggaagaaa actggagtgg
      661 ggtatccaca gctcagcgca gtgatggagt gtgcagatgc tgctcatggc ctcaaaggcc
      721 acatcatttc agatggaggt tgcagctgtc ctggggatgt ggccaaggct tttggggcag
      781 gagctgactt cgtgatgctg ggtggcatgc tggctgggca cagtgagtca ggtggtgagc
      841 tcatcgagag ggatggcaag aagtacaagc tcttctatgg aatgagttct gaaatggcca
      901 tgaagaagta tgctgggggc gtggctgagt acagagcctc
//
