LOCUS       sequence-59286-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FABP1A
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             561..578
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(631..649)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      645..692
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(816..835)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgagt
      121 ttctccggca agtaccaact gcagagccag gaaaactttg aagccttcat gaaggcaatc
      181 ggtctgccgg aagagctcat ccagaagggg aaggatatca agggggtgtc ggaaatcgtg
      241 cagaatggga agcacttcaa gttcaccatc accgctgggt ccaaagtgat ccaaaacgaa
      301 ttcacggtgg gggaggaatg tgagctggag acaatgacag gggagaaagt caagacagtg
      361 gttcagttgg aaggtgacaa taaactggtg acaactttca aaaacatcaa gtctgtgacc
      421 gaactcaacg gcgacataat caccaatacc atgacattgg gtgacattgt cttcaagaga
      481 atcagcaaga gaatttgaca gtaaaggtgg atacggatcc gaattcgagc tccgtcgaca
      541 agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag
      601 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      661 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg
      721 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      781 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
      841 tctcgccacg ttcgccggct ttcccgtcaa gctctaaatc gggggctccc tttagggttc
      901 cgatttagtg ctttacggca cctcgacccc aaaaacttga ttagggtgat ggttc
//
