LOCUS       sequence-59287-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ITPAA
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..104
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             716..733
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(786..804)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcacagc agcggcctgg ttccgcgtgg tagtatggcg gcctcattgg
      121 tggggaagaa gatcgtgttt gtaacgggga acgccaagaa gctggaggag gtcgttcaga
      181 ttctaggaga taagtttcca tgcactttgg tggcacagaa aattgacctg ccggagtacc
      241 agggggagcc ggatgagatt tccatacaga aatgtcagga ggcagttcgc caggtacagg
      301 ggcccgtgct ggttgaggac acttgtctgt gcttcaatgc ccttggaggg ctccccggcc
      361 cctacataaa gtggtttctg gagaagttaa agcctgaagg tctccaccag ctcctggccg
      421 ggttcgagga caagtcagcc tatgcgctct gcacgtttgc actcagcacc ggggacccaa
      481 gccagcccgt gcgcctgttc aggggccgga cctcgggccg gatcgtggca cccagaggct
      541 gccaggactt tggctgggac ccctgctttc agcctgatgg atatgagcag acgtacgcag
      601 agatgcctaa ggcggagaag aacgctgtct cccatcgctt ccgggccctg ctggagctgc
      661 aggagtactt tggcagtttg gcagcttgat gacgaagctt gcggccgcac tcgagcacca
      721 ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      781 tgccaccgct gagcaataac tagcataacc cccttggggc ctctaaacgg ggtcttgagg
      841 ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg
      901 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gacgctacac ttgc
//
