LOCUS       sequence-59288-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TULP1A
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             927..944
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatggaa
      121 ccccgggagt ttgtgctccg gcctgccccc cagggccgca cggtgcgctg ccggctgacc
      181 cgggacaaaa agggcatgga tcgaggcatg tatccctcct acttcctgca cctggacacg
      241 gagaagaagg tgttcctctt ggctggcagg aaacgaaaac ggagcaagac agccaattac
      301 ctcatctcca tcgaccctac caatctgtcc cgaggagggg agaatttcat cgggaagctg
      361 aggtccaacc tcctggggaa ccgcttcacg gtctttgaca acgggcagaa cccacagcgt
      421 gggtacagca ctaatgtggc aagccttcgg caggagctgg cagctgtgat ctatgaaacc
      481 aacgtgctgg gcttccgtgg cccccggcgc atgaccgtca tcattcctgg catgagtgcg
      541 gagaacgaga gggtccccat ccggccccga aatgctagtg acggcctgct ggtgcgctgg
      601 cagaacaaga cgctggagag cctcatagaa ctgcacaaca agccacctgt ctggaacgat
      661 gacagtggct cctacaccct caacttccaa ggccgggtca cccaggcctc agtcaagaac
      721 ttccagattg tccacgctga tgaccccgac tatatcgtgc tgcagttcgg ccgcgtggcg
      781 gaggacgcct tcaccctaga ctaccggtac ccgctgtgcg ccctgcaggc cttcgccatc
      841 gccctctcca gtttcgacgg gtgacagtaa aggtggatac ggatccgaat tcgagctccg
      901 tcgaaaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta
      961 acaaagcc
//
