LOCUS       sequence-59289-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IMPA2A
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..39
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             92..112
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tccctctaga aataattttg tttaacttta agaaggagat atacatatgc accatcatca
       61 tcatcattct tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgaagcc
      121 gagcggcgag gaccaggcgg cgctggcggc cggcccctgg gaggagtgct tccaggcggc
      181 cgtgcagctg gcgctgcggg caggacagat catcagaaaa gcccttactg aggaaaaacg
      241 tgtctcaaca aaaacatcag ctgcagatct tgtgacagaa acagatcacc ttgtggaaga
      301 tttaattatt tctgagttgc gagagaggtt tccttcacac aggttcattg cagaagaggc
      361 cgcggcttct ggggccaagt gtgtgctcac ccacagcccg acgtggatca tcgaccccat
      421 cgacggcacc tgcaattttg tgcacagatt cccgactgtg gcggttagca ttggatttgc
      481 tgttcgacaa gagcttgaat tcggagtgat ttaccactgc acagaggagc ggctgtacac
      541 gggccggcgg ggtcggggcg ccttctgcaa tggccagcgg ctccgggtct ccggggagac
      601 agatctctca aaggccttgg ttctgacaga aattggcccc aaacgtgacc ctgcgaccct
      661 gaagctgttc ctgagtaaca tggagcggct gctgcatgcc aaggcgcatg gggtccgagt
      721 gattggaagc tccacattgg cactctgcca cctggcctca ggggccgcgg atgcctatta
      781 ccagtttggc ctgcactgct gggatctggc ggctgccaca gtcatcatca gagaagcagg
      841 cggcatcgtg atagacactt cgggtggacc cctcgacctc atggcttgca gagtggttgc
      901 ggccagcacc cgggagatgg cgatgctcat agctcaggcc ttacagacga t
//
