LOCUS       sequence-59293-e         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DDX2AA
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             840..857
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(910..928)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatggaa
      121 ggcgtcatcg agagtaactg gaatgagatt gttgacagct ttgatgacat gaacctctcg
      181 gagtcccttc tccgtggcat ctacgcctat ggttttgaga agccctctgc catccagcag
      241 cgagccattc taccttgtat caagggttat gatgtgattg ctcaagccca atctgggact
      301 gggaaaacgg ccacatttgc catatcaatt ctgcagcaga ttgaattaga tctaaaagcc
      361 acccaggcct tggtcctagc acccactcga gaattggctc agcagataca gaaggtggtc
      421 atggcactag gagactacat gggcgcctcc tgtcacgcct gtatcggggg caccaacgtg
      481 cgtgctgagg tgcagaaact gcagatggaa gctccccaca tcatcgtggg tacccctggc
      541 cgtgtgtttg atatgcttaa ccggagatac ctgtccccca aatacatcaa gatgtttgta
      601 ctggatgaag ctgacgaaat gttaagccgt ggattcaagg accagatcta tgacatattc
      661 caaaagctca acagcaacac ccaggtagtt ttgctgtcag ccacaatgcc ttctgatgtg
      721 cttgaggtga ccaagaagtt catgagggac cccattcgga ttcttgtcaa gaagtgacag
      781 taaaggtgga tacggatccg aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc
      841 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      901 ctgctgccac cgctgagcaa taactagcat aaccctgggg gcctctaaac gggtcttgag
      961 ggttttttgc tgaaaggagg actatatccg gattgg
//
