LOCUS       sequence-59294-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Lentini R, Forlin M, Martini L, Del Bianco C, Spencer AC, Torino D, 
            Mansy SS
  TITLE     Fluorescent Proteins and in Vitro Genetic Organization for Cell-Free
            Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2013 Mar 15.
  PUBMED    23654270
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Mar 15."
COMMENT     Sequence Label: pET21b-RL020A
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..28)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             753..775
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccggatctca gtggtggtgg tggtggtgct cgagttattt atacagctca tccataccac
       61 cggtagaatg acgtccttca gcacgctcat actgctccac gatcgtatag tcctcattgt
      121 gtgaggtgat gtccagtttg atgttaacgt tataggcacc aggcagttgg acaggttttt
      181 tggctttata cgtggttttc acttcggcat catagtggcc accatctttc agtttcagac
      241 gctgtttgat ttcgcctttc agagcgccat cttctggata catacgctca ctagaggcct
      301 cccaacccat cgtttttttc tgcataaccg ggccatcact cggaaaattc gtgccacgca
      361 gtttcacttt atagatgaac tcaccgtctt gcaggctaga atcctgtgtc actgtcacaa
      421 caccaccatc ctcaaagttc atcacacgtt cccatttgaa cccttcaggg aagctcagtt
      481 tcagatagtc cggaatatcg gccggatgtt tcacataggc tttggagcca tacatgaact
      541 gtgggctcag aatatcccaa gcaaatggca gtggaccacc tttcgtgact ttcagtttag
      601 cggtctgtgt accctcatac ggacgacctt cgccttcgcc ctcaatttcg aactcgtgac
      661 cattaacgct gccttccatg tggactttga agcgcatgaa ctctttgatg atcgccatat
      721 tgtcctcctc gcctttactc accatatgta tatctccttc ttaaagttaa acaaaattat
      781 tcggatccgc ttatttatac agttcatcca taccatgcgt aa
//
