LOCUS       sequence-59295-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PIP5K2CA
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgcat
      121 ttcgtgcagc agaaggtgaa ggtgttccgg gcggccgacc cgctggtggg tgtgttcctg
      181 tggggcgtag cccactcgat caatgagctc agccaggtgc ctcccccggt gatgctgctg
      241 ccagatgact ttaaggccag ctccaagatc aaggtcaaca atcacctttt ccacagggaa
      301 aatctgccca gtcatttcaa gttcaaggag tattgtcccc aggtcttcag gaacctccgt
      361 gatcgatttg gcattgatga ccaagattac ttggtgtccc ttacccgaaa cccccccagc
      421 gaaagtgaag gcagtgatgg tcgcttcctt atctcctacg atcggactct ggtcatcaaa
      481 gaagtatcca gtgaggacat tgctgacatg catagcaacc tctccaacta tcaccagtac
      541 attgtgaagt gccatggcaa cacgcttctg ccccagttcc tggggatgta ccgagtcagt
      601 gtggacaacg aagacagcta catgcttgtg atgcgcaata tgtttagcca ccgtcttcct
      661 gtgcacagga agtatgacct caagggttcc ctagtgtccc gggaagccag cgataaggaa
      721 aaggttaaag aattgcccac ccttagggat atggactttc tcaacaagaa ccagaaagta
      781 tatattggtg aagaggagaa gaaaatattt ctggagaagc tgaagagaga tgtggagttt
      841 ctagtgcagc tgaagatcat ggactacagc cttctgctag gcatccacga catcattcgg
      901 ggctctgaac cagaggagga agcgcccgtg cgggaggatg agtca
//
