LOCUS       sequence-59299-e        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DRP2A
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgacg
      121 agcgatcgtc ttctgatcaa aggaggtaaa attgttaatg atgaccagtc gttctatgca
      181 gacatataca tggaagatgg gttgatcaag caaataggag aaaatctgat tgtgccagga
      241 ggagtgaaga ccatcgaggc ccactcccgg atggtgatcc ccggaggaat tgacgtccac
      301 actcgtttcc agatgcctga tcagggaatg acgtctgctg atgatttctt ccaaggaacc
      361 aaggcggccc tggctggggg aaccactatg atcattgacc acgttgttcc tgagcctggg
      421 acaagcctgc tcgctgcctt tgaccagtgg agggaatggg ccgacagcaa gtcctgctgt
      481 gactactctc tgcatgtgga catcagcgag tggcataagg gcatccagga ggagatggaa
      541 gcgcttgtga aggatcacgg ggtaaattcc ttcctcgtgt acatggcttt caaagatcgc
      601 ttccagctaa cggattgcca gatttatgaa gtactgagtg tgatccggga tattggcgcc
      661 atagcccaag tccacgcaga aaatggcgac atcattgcag aggagcagca gaggatcctg
      721 gatctgggca tcacgggccc cgagggacat gtgctgagcc gacctgagga ggtcgaggcc
      781 gaagccgtga atcgtgccat caccatcgcc aaccagacca actgcccgct gtatatcacc
      841 aaggtgatga gcaaaagctc tgctgaggtc atcgcccagg cacggaagaa gggaactgtg
      901 gtgtatggcg agcccatcac tgccagcttg ggaacggacg gctcccatta ctggagcaag
      961 aactgggcca aggctgctgc ctttgtcacc tccccaccct tgagcc
//
