LOCUS       sequence-59300-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  TITLE     Howard Steinman lab unpublished sacB bacterial exchange vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSR47S Cm
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(33..135)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     368..387
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
      121 cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca
      181 acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg
      241 gcgcaaagtg cgtcgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg
      301 aggccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga cgggcgcccc
      361 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
      421 ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt
      481 ggctttcccc ccccccccga tccggccacg atgcgtccgg cgtagaggat ctgaagatca
      541 gcagttcaac ctgttgatag tacgtactaa gctctcatgt ttcacgtact aagctctcat
      601 gtttaacgta ctaagctctc atgtttaacg aactaaaccc tcatggctaa cgtactaagc
      661 tctcatggct aacgtactaa gctctcatgt ttcacgtact aagctctcat gtttgaacaa
      721 taaaattaat ataaatcagc aacttaaata gcctctaagg
//
