LOCUS       sequence-59302-e         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NT5C3A
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..104
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcacagc agcggcctgg ttccgcgtgg tagtaaccct acaagagtag
      121 aagaaattat ctgtggtctt atcaaaggag gagctgccaa acttcagata ataacggact
      181 ttgatatgac actcagtaga ttttcatata aagggaaaag atgcccaaca tgtcataata
      241 tcattgacaa ctgtaagctg gttacagatg aatgtagaaa aaagttattg caactaaagg
      301 aaaaatacta cgctattgaa gttgatcctg ttcttactgt agaagagaag tacccttata
      361 tggtggaatg gtatactaaa tcacatggtt tgcttgttca gcaagcttta ccaaaagcta
      421 aacttaaaga aattgtggca gaatctgacg ttatgctcaa agaaggatat gagaatttct
      481 ttgataagct ccaacaacat agcatccccg tgttcatatt ttcggctgga atcggcgatg
      541 tactagagga agttattcgt caagctggtg tttatcatcc caatgtcaaa gttgtgtcca
      601 attttatgga ttttgatgaa actggggtgc tcaaaggatt taaaggagaa ctaattcatg
      661 tatttaacaa acatgatggt gccttgagga atacagaata tttcaatcaa ctaaaagaca
      721 atagtaacat aattcttctg ggagactccc aaggagactt aagaatggca gatggagtgg
      781 ccaatgttga gcacattctg aaaattggat atctaaatga tagagtggat gagcttttag
      841 aaaagtacat ggactcttat gatattgttt tagtacaaga tgaatcatta gaagtagcca
      901 actctatttt acagaagatt ctatgatgac gaagcttgcg gccgcactcg agcaccacac
      961 ccacaccact gag
//
