LOCUS       sequence-59303-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: THEM2A
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             549..566
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(619..637)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      633..680
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(804..823)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgcgc
      121 aattttgaga gagttttggg aaagattact cttgtctctg ctgctcctgg gaaagtgatt
      181 tgtgaaatga aagtagaaga agagcatacc aatgcaatag gcactctcca cggcggtttg
      241 acagccacgt tagtagataa catatcaaca atggctctgc tatgcacgga aaggggagca
      301 cccggagtca gtgtcgatat gaacataacg tacatgtcac ctgcaaaatt aggagaagat
      361 atagtgatta cagcacatgt tctgaagcaa ggaaaaacac ttgcatttac ctctgtggat
      421 ctgaccaaca aggccacagg aaaattaata gcacaaggaa gacacacaaa acacctggga
      481 aactgacagt aaaggtggat acggatccga attcgagctc cgtcgacaag cttgcggccg
      541 cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag
      601 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      661 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc
      721 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
      781 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      841 cgccggcttt ccccgtcagg ctctaaatcg ggggctccct tttagggttc cgatttagtg
      901 ctttacggca cctcgacccc aaaaacttga ttagggtgga tggttcacgt agtgggccat
      961 cgccctgata
//
