LOCUS       sequence-59305-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SCLYA
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgggg
      121 agggatgcgc cggcacccgc ggcgagtcag cccagcggct gcgggaaaca caactcgccg
      181 gagaggaaag tttatatgga ctataatgca acgactcccc tggagccaga agttatccag
      241 gccatgacca aggccatgtg ggaagcctgg ggaaatccca gcagcccgta ttcagcagga
      301 agaaaggcca aggatattat aaatgcagct cgggaaagcc tcgcgaagat gatagggggg
      361 aaacctcaag atataatctt cacttccggg ggcactgagt caaataattt agtaatccat
      421 tctgtggtga aacatttcca cgcaaaccag acctcaaagg gacacacagg tgggcaccac
      481 agcccagtga agggggccaa gccccatttc attacttcct cggtggaaca cgactccatc
      541 cggctgcccc tggagcacct ggtggaagaa caagtggcag cggtcacctt tgtcccggtg
      601 tccaaggtga gcgggcagac agaggtggac gacatcctcg cggcagtccg cccgaccaca
      661 cgcctcgtga ccatcatgct ggccaacaat gagactggca ttgtcatgcc tgtccctgaa
      721 atcagtcagc gcattaaagc cctgaaccag gaacgggtgg cagctgggct acctcccatc
      781 ctcgtgcaca cggatgctgc acaggccttg gggaagcagc gcgtggatgt ggaggacctg
      841 ggcgtggact tccttacaat cgtggggcac aagttttatg gtcccaggat tggcgcactt
      901 tatata
//
