LOCUS       sequence-59309-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Houser JR, Ford E, Chatterjea SM, Maleri S, Elston TC, Errede B
  TITLE     An improved short-lived fluorescent protein transcriptional reporter
            for Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):519-30. doi: 10.1002/yea.2932. Epub 2012 Nov 
            21.
  PUBMED    23172645
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2932"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "519-30"
COMMENT     Sequence Label: pNC1125
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(64..774)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(104..124)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     719..747
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 acttatttat aataaaaatc ataaatcata agaaattcgc ttatttagaa gtggcgcgcc
       61 ctatttgtat agttcatcca tgccatgtgt aatcccagca gctgttacaa actcaagaag
      121 gaccatgtgg tctctctttt cgttgggatc tttcgaaagg gcagattgtg tggacaggta
      181 atggttgtct ggtaaaagga cagggccatc gccaattgga gtattttgtt gataatggtc
      241 tgctagttga acgcttccat cttcaatgtt gtgtctaatt ttgaagttag ctttgattcc
      301 attcttttgt ttgtctgcca tgatgtatac attgtgtgag ttatagttgt attccaattt
      361 gtgcccaaga atgtttccat cttctttaaa atcaatacct tttaactcga ttctattaac
      421 aagggtatca ccttcaaact tgacttcagc acgtgtcttg tagttcccgt catctttgaa
      481 aaatatagtt ctttcctgta cataaccttc gggcatggca ctcttgaaaa agtcatgctg
      541 tttcatgtga tctgggtatc ttgaaaagca ttgaacacca taagtgagag tagtgacaag
      601 tgttggccat ggaacaggta gttttccagt agtgcaaata aatttaaggg taagttttcc
      661 gtatgttgca tcaccttcac cctctccact gacagaaaat ttgtgcccat taacatcacc
      721 atctaattca acaagaattg ggacaactcc agtgaaaagt tcttctcctt tactcgtatt
      781 gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagccaag ctccggancg
      841 gaacacctac taccaagagg acctcgagga c
//
