LOCUS       sequence-59310-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Houser JR, Ford E, Chatterjea SM, Maleri S, Elston TC, Errede B
  TITLE     An improved short-lived fluorescent protein transcriptional reporter
            for Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):519-30. doi: 10.1002/yea.2932. Epub 2012 Nov 
            21.
  PUBMED    23172645
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2932"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "519-30"
COMMENT     Sequence Label: pNC1125
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(439..769)
                     /gene="S. cerevisiae GAL10"
                     /label=GAL10 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    652..769
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 attcgaattc gagctctgga gaatatacta agggtactgt tgacattgcg aagagcgaca
       61 aagattttgt tatcggcttt attgctcaaa gagacatggg tggaagagat gaaggttacg
      121 attggttgat tatgacaccc ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac
      181 agtatagaac cgtggatgat gtggtctcta caggatctga cattattatt gttggaagag
      241 gactatttgc aaagggaagg gatgctaagg tagagggtga acgttacaga aaagcaggct
      301 gggaagcata tttgagaaga tgcggccagc aaaactaaaa aactgtatta taagtaaatg
      361 catgtatact aaactcacaa attagagctt caatttaatt atatcagtta ttacccggta
      421 cccccctcga ggaattttca aaaattctta cttttttttt ggatggacgc aaagaagttt
      481 aataatcata ttacatggca ttaccaccat atacatatcc atatacatat ccatatctaa
      541 tcttacttat atgttgtgga aatgtaaaga gccccattat cttagcctaa aaaaaccttc
      601 tctttggaac tttcagtaat acgcttaact gctcattgct atattgaagt acggattaga
      661 agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt cctcgtcttc
      721 accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga acaataaaga
      781 ttctacaata ctagctttta tggttatg
//
