LOCUS       sequence-59313-e        6268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Talbot JJ, Shillingford JM, Vasanth S, Doerr N, Mukherjee S, Kinter 
            MT, Watnick T, Weimbs T
  TITLE     Polycystin-1 regulates STAT activity by a dual mechanism.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 May 10;108(19):7985-90. Epub 2011 Apr
            25.
  PUBMED    21518865
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2011-05-10"; volume: "108(19)"; pages: 
            "7985-90. Epub 2011 Apr 25"
COMMENT     Sequence Label: NTM (FLM-PC1-Delta-137)
FEATURES             Location/Qualifiers
     source          1..6268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1174..1203
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1213..1242
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1258..1275
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1298..1315)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1304..1528
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1574..2002
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1661..1680)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1871..1892
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2011..2031)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2016..2346
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2197..2332
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2259..2278
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        2394..2441
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2460..2834
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    2964..3085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3001..3020)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3055..3074
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3134..3150)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3134..3150)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3147..3169)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3158..3174
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3182..3188,3189..3206,3207..3212))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3227..3248
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3365..3382)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3536..4124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3616..3635)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4295..5086,5087..5155))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4918..4937
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5156..5260)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5335..5354)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5526..5905
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5906..6109
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6060..6080
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    6111..6129
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6132..6150
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6160..6184
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 aagcttctag agatccctcg acctcgagat ccattgtgct ctaaagatct ttggtgactt
       61 gtccactcca gtgtggcatc atgtggcagc tgctcctccc aactgctctg ctacttctag
      121 tttcagctgg catgcggact gaagatctcc caaaggctgt ggtgttcctg gagcctcaat
      181 ggtacagcgt gcttgagaag gacagtgtga ctctgaagtg ccagggagcc tactcccctg
      241 aggacaattc cacacagtgg tttcacaatg agagcctcat ctcaagccag gcctcgagct
      301 acttcattga cgctgccaca gtcaacgaca gtggagagta caggtgccag acaaacctct
      361 ccaccctcag tgacccggtg cagctagaag tccatatcgg ctggctgttg ctccaggccc
      421 ctcggtgggt gttcaaggag gaagacccta ttcacctgag gtgtcacagc tggaagaaca
      481 ctgctctgca taaggtcaca tatttacaga atggcaaaga caggaagtat tttcatcata
      541 attctgactt ccacattcca aaagccacac tcaaagatag cggctcctac ttctgcaggg
      601 ggcttgttgg gagtaaaaat gtgtcttcag agactgtgaa catcaccatc actcaaggtt
      661 tggcagtgtc aaccatctca tcattctctc cacctggcgc ggatccacca agggcctctg
      721 ccctccctgc cccaccgaca ggctccgccc tccctgaccc gcagacagcc tctgccctcc
      781 ctgacccgcc agcagcctct gccctccctg cggccctggc ggtgatctcc ttcctcctcg
      841 ggctgggcct gggggtggcg tgtgtgctgg cgaggacgcg tgagtcctgg cacttgtcac
      901 ccctgctgtg tgtggggctc tgggcactgc ggctgtgggg cgccctacgg ctgggggctg
      961 ttattctccg ctggcgctac cacgccttgc gtggagagct gtaccggccg gcctgggagc
     1021 cccaggacta cgagatggtg gagttgttcc tgcgcaggct gcgcctctgg atgggcctca
     1081 gcaaggtcaa ggagttccgc cacaaagtcc gctttgaagg gatggagccg ctgccctctc
     1141 gctcctccag gggctcctag ggcggccgct cgagaacaaa aactcatctc agaagaggat
     1201 ctggggccct tcgaacaaaa actcatctca gaagaggatc tgaatatgca taccggtcat
     1261 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     1321 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     1381 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     1441 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     1501 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     1561 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     1621 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     1681 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta
     1741 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     1801 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     1861 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     1921 tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt
     1981 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     2041 ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2101 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     2161 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     2221 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     2281 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     2341 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     2401 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     2461 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     2521 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     2581 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     2641 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     2701 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     2761 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     2821 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     2881 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     2941 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     3001 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3061 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     3121 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     3181 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3241 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     3301 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     3361 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3421 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3481 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3541 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3601 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3661 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3721 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3781 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3841 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3901 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3961 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4021 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4081 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4141 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4201 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4261 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4321 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4381 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4441 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4501 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4561 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     4621 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4681 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     4741 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4801 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4861 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     4921 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     4981 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5041 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5101 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5161 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5221 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5281 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat
     5341 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     5401 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     5461 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
     5521 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     5581 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     5641 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     5701 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     5761 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     5821 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     5881 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     5941 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     6001 gttttggaac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     6061 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag
     6121 tgatagagat ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca
     6181 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
     6241 cagcctccgg actctagcgt ttaaactt
//
