LOCUS       sequence-59327-e        8978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8978)
  AUTHORS   Liu DS, Loh KH, Lam SS, White KA, Ting AY
  TITLE     Imaging trans-cellular neurexin-neuroligin interactions by enzymatic
            probe ligation.
  JOURNAL   PLoS One. 2013;8(2):e52823. doi: 10.1371/journal.pone.0052823. Epub 
            2013 Feb 14.
  PUBMED    23457442
REFERENCE   2  (bases 1 to 8978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(2):e52823. doi"; pages: " 
            10.1371/journal.pone.0052823. Epub 2013 Feb 14"
COMMENT     Sequence Label: pXL-CAG-LAP2-NLG1
FEATURES             Location/Qualifiers
     source          1..8978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   746..808
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1072..1375
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1378..1653
                     /label=chicken beta-actin promoter
     intron          1654..2663
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2587..2609
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2671..2690
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2730..2754
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3512..3521
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5477..5501)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5592..5611)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5657..5712
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5711..5730)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   6304..6338
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6829..6845)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6829..6845)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6842..6864)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6853..6869
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6877..6883,6884..6901,6902..6907))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6922..6943
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7060..7077)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7231..7819)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7311..7330)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7990..8781,8782..8850))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8613..8632
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8851..8955)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg
      661 gcagactcgc ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac
      721 gctgcagaac acgcagctag attaacccta gaaagataat catattgtga cgtacgttaa
      781 agataatcat gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt
      841 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc
      901 aacaaacaat ttatttatgt ttatttattt attaaaaaaa aacaaaaact caaaatttct
      961 tctataaagt aacaaaactt ttatcgaatt cctgcagccc gggggatcca ctagttatta
     1021 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     1081 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     1141 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     1201 ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     1261 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     1321 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatgggtcg
     1381 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
     1441 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc
     1501 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg
     1561 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
     1621 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt
     1681 gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
     1741 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
     1801 cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga gggccctttg
     1861 tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
     1921 cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
     1981 cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga
     2041 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     2101 ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     2161 cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     2221 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     2281 aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     2341 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctggcggag
     2401 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg
     2461 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     2521 tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca
     2581 gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc
     2641 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
     2701 attttggcaa agaattcctc gaggatatca caagtttgta caaaaaagca ggctccgaat
     2761 tcgcccttcg taactataac ggtcctaagg tagcgaatag tgaaccgtca gatccgctag
     2821 cgctaccgga ctcagatctc gagctcaagc ttgggaccat ggcacttccc agatgcatgt
     2881 ggccaaatta tgtttggaga gctatgatgg catgtgtggt ccacagggga tccggtgccc
     2941 cattgactct ctgcttgttg ggatgtttgc tacagacttt tcacgtactc tctcaaaagt
     3001 atcgatcggg attcgagatc gacaaggtgt ggtacgacct ggacgccgtc gacgcattgg
     3061 atgatgtaga cccattggtt actactaact ttggcaagat taggggaatt aagaaagaac
     3121 tcaataatga aattttgggt cctgtcattc agtttcttgg ggttccatat gccgctccac
     3181 caacaggaga acatcgtttc cagcctccag aaccaccatc tccctggtct gacatccgga
     3241 acgccactca gtttgctcct gtatgtcccc agaatatcat tgatggcaga ttgcctgaag
     3301 ttatgcttcc tgtgtggttc actaataact tggatgtggt ttcatcatac gtccaagacc
     3361 agagtgaaga ctgtctatac ttaaacatct atgtcccaac tgaagatggt ccccttacaa
     3421 agaaacacac agatgattta ggtgataatg acggtgctga agatgaagat attcgggaca
     3481 gtgggggtcc caaaccagtg atggtgtaca tccatggcgg ctcttacatg gaaggtactg
     3541 gaaatctgta tgatgggagt gtcttggcaa gctatggcaa tgtgatcgtc atcacagtca
     3601 actatcggct tggggtactt ggcttcttga gcacagggga tcaggctgcc aaaggaaact
     3661 acgggctcct tgacctcatc caggccctaa gatggaccag cgagaacatt gggttctttg
     3721 gtggtgaccc cttgcgaatc actgtgtttg gatcaggcgc tgggggttca tgtgtcaacc
     3781 tgctgacttt atcccattat tctgaaggta accgttggag caattcaacc aaaggacttt
     3841 ttcaacgagc aatagctcag agtggaacag ccctttccag ctgggctgtt agtttccagc
     3901 ctgcaaaata cgctagaatt ctggccacaa aagttggctg caatgtttca gatacagtag
     3961 agttagtaga atgcctgcag aagaagcctt acaaagaact tgttgatcaa gatgttcaac
     4021 cagcccgata ccacatagcc tttggacctg tgatcgatgg tgatgtaata ccagatgacc
     4081 ctcagatact gatggaacaa ggagagttcc tcaactatga tataatgttg ggagttaacc
     4141 aaggggaagg gttgaagttt gtcgaaaata tagtagatag tgatgatggt gtatcagcca
     4201 gtgatttcga ctttgctgtt tctaattttg ttgataattt atatggatat cctgaaggca
     4261 aagatgtttt gagagaaacc attaaattca tgtatactga ctgggctgat cgccataatc
     4321 ctgaaactag aaggaagaca ttgttggctt tgtttacgga ccatcaatgg gtagcacctg
     4381 ctgtggccac agcagacctt cactcgaact ttggctcacc tacatacttc tatgcctttt
     4441 atcatcattg ccaaacagat caagttccag cttgggctga tgcagctcat ggggatgagg
     4501 ttccctatgt gttgggaatc cccatgattg gccctacaga gttatttcct tgcaatttct
     4561 ccaagaatga tgtgatgttg agtgcagtag taatgacata ctggacgaat tttgctaaaa
     4621 ctggtgaccc aaatcaacca gttcctcaag acacaaaatt catccatacc aaacccaacc
     4681 gctttgaaga agtagcatgg accagatatt cccagaaaga ccagctttat ctccatattg
     4741 gattaaaacc gagagttaaa gagcattaca gagccaataa ggtaaatctc tggctggagc
     4801 tggtacctca tctgcataat ctcaatgaca tttctcagta tacctcgaca acaactaaag
     4861 tgccatccac ggacatcact ctcagaccta caaggaagaa ttccacacca gtcacatcag
     4921 cctttcccac tgccaaacag gatgatccca agcaacaacc aagccccttc tcggtggatc
     4981 agagggacta ctccacagag ctaagtgtca ctatcgcagt gggggcctct ctgctgtttc
     5041 tcaacatctt ggcttttgca gccctgtact acaagaagga taagaggaga catgatgtcc
     5101 accggaggtg cagccctcag cgcacgacca ccaacgacct aacccatgct ccagaagagg
     5161 aaattatgtc tctccaaatg aagcacactg acttggatca cgagtgtgag tccatccatc
     5221 cacatgaggt ggttcttcgg accgcctgtc ccccagatta tactctagct atgaggaggt
     5281 cacctgatga tattccacta atgacaccta acaccatcac aatgattccc aacactatac
     5341 cagggattca gcccttacat acattcaaca catttactgg aggacagaat aatacactgc
     5401 cccatcccca cccacacccc cattcacatt caacaaccag ggtatagaag ctagcggggg
     5461 gccaagggcg aattcgaccc agctttcttg tacaaagtgg tgattcgcgg ccgcactcct
     5521 caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa
     5581 taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
     5641 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     5701 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     5761 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaagttgct
     5821 ataagaggtc atcagtatat gaaacagccc ctgctgtcca ttctatccat agaaagctga
     5881 ctgagtagat ttttttatat ttgtttgtgt atttttctac atccctaaat ttcnacatgt
     5941 ttactagcga attttcctcc ctctgactac tcccagtcat agctgtccct cttctcttat
     6001 gaagatccct cgacctgcag cccaagcttg atatctataa caagaaaata tatatataat
     6061 aagttatcac gtaagtagaa catgaaataa caatataatt atcgtatgag ttaaatctta
     6121 aaagtcacgt aaaagataat catgcgtcat tttgactcac gcggtcgtta tagttcaaaa
     6181 tcagtgacac ttaccgcatt gacaagcacg cctcacggga gctccaagcg gcgactgaga
     6241 tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa gagagagcaa tatttcaaga
     6301 atgcatgcgt caattttacg cagactatct ttctagggtt aatctagctg catcaggatc
     6361 atatcgtcgg gtcttttttc cggctcagtc atcgcccaag ctggcgctat ctgggcatcg
     6421 gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc ggcatggaaa gagtttgccg
     6481 aggatgactg ctgctgcatt gacgttgagc gaaaacgcac gtttaccatg atgattcggg
     6541 aaggtgtggc catgcacgcc tttaacggtg aactgttcgt tcaggccacc tgggatacca
     6601 gttcgtcgcg gcttttccgg acacagttcc ggatggtcag cccgaagcgc atcagcaacc
     6661 cgaacaatac cggcgacagc cggaactgcc gtgccggtgt gcagattaat gacagcggtg
     6721 cggcgctggg atattacgtc agcgaggacg ggtatcctgg ctggatgccg cagaaatgga
     6781 catggatacc ccgtgagtta cccggcgggc gcgcttggcg taatcatggt catagctgtt
     6841 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6901 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6961 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7021 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7081 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7141 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7201 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7261 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7321 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7381 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7441 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7501 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7561 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7621 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     7681 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7741 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7801 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7861 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7921 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7981 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8041 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8101 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8161 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8221 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8281 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8341 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8401 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8461 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8521 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8581 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8641 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8701 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8761 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8821 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8881 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8941 aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
