LOCUS       sequence-59333-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Houser JR, Ford E, Chatterjea SM, Maleri S, Elston TC, Errede B
  TITLE     An improved short-lived fluorescent protein transcriptional reporter
            for Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):519-30. doi: 10.1002/yea.2932. Epub 2012 Nov 
            21.
  PUBMED    23172645
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2932"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "519-30"
COMMENT     Sequence Label: pNC1136
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(54..764)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(94..114)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     709..737
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aataaaaatc ataaatcata agaaattcgc ttatttagaa gtggcgcgcc ctatttgtat
       61 agttcatcca tgccatgtgt aatcccagca gctgttacaa actcaagaag gaccatgtgg
      121 tctctctttt cgttgggatc tttcgaaagg gcagattgtg tggacaggta atggttgtct
      181 ggtaaaagga cagggccatc gccaattgga gtattttgtt gataatggtc tgctagttga
      241 acgcttccat cttcaatgtt gtgtctaatt ttgaagttag ctttgattcc attcttttgt
      301 ttgtctgcca tgatgtatac attgtgtgag ttatagttgt attccaattt gtgcccaaga
      361 atgtttccat cttctttaaa atcaatacct tttaactcga ttctattaac aagggtatca
      421 ccttcaaact tgacttcagc acgtgtcttg tagttcccgt catctttgaa aaatatagtt
      481 ctttcctgta cataaccttc gggcatggca ctcttgaaaa agtcatgctg tttcatgtga
      541 tctgggtatc ttgaaaagca ttgaacacca taagtgagag tagtgacaag tgttggccat
      601 ggaacaggta gttttccagt agtgcaaata aatttaaggg taagttttcc gtatgttgca
      661 tcaccttcac cctctccact gacagaaaat ttgtgcccat taacatcacc atctaattca
      721 acaagaattg ggacaactcc agtgaaaagt tcttctcctt tactcgta
//
