LOCUS       sequence-59334-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Lentini R, Forlin M, Martini L, Del Bianco C, Spencer AC, Torino D, 
            Mansy SS
  TITLE     Fluorescent Proteins and in Vitro Genetic Organization for Cell-Free
            Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2013 Mar 15.
  PUBMED    23654270
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Mar 15."
COMMENT     Sequence Label: pET21b-RL020A
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             755..777
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 actttaagaa ggagatatac atatgagcaa aggcgaagaa ctgttcacgg gtgtggttcc
       61 gatcctggtt gaactggatg gcgatgtgaa cggtcataaa tttagcgtgt ctggtgaagg
      121 cgaaggtgat gcgacctacg gcaaactgac gctgaaactg atttgcacca cgggtaaact
      181 gccggttccg tggccgaccc tggtgaccac gctgggttat ggtctgatgt gtttcgcacg
      241 ttacccggat cacatgaaac gccatgattt ctttaaatct gcgatgccgg aaggctatgt
      301 gcaggaacgt accatctttt tcaaagatga tggtaactac aaaacccgcg cggaagttaa
      361 atttgaaggc gatacgctgg tgaaccgtat tgaactgaaa ggtatcgatt tcaaagaaga
      421 tggcaatatt ctgggtcaca aactggaata caactacaac agtcataacg tgtacattac
      481 cgccgataaa cagaaaaacg gtatcaaagc aaacttcaaa atccgtcaca acatcgaaga
      541 tggcggtgtt cagctggccg atcattacca gcagaacacc ccgattggcg atggtccggt
      601 gctgctgccg gataatcatt atctgagtta ccagagcaaa ctgtctaaag atccgaatga
      661 aaaacgcgat cacatggttc tgctggaatt tgtgaccgcg gccggcatta cgcatggtat
      721 ggatgaactg tataaataag cggatccgaa taattttgtt taactttaag aaggagatat
      781 acatatggtg agtaaaggcg aggaggacaa tatggcgatc atc
//
