LOCUS       sequence-59336-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Houser JR, Ford E, Chatterjea SM, Maleri S, Elston TC, Errede B
  TITLE     An improved short-lived fluorescent protein transcriptional reporter
            for Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2012 Dec;29(12):519-30. doi: 10.1002/yea.2932. Epub 2012 Nov 
            21.
  PUBMED    23172645
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2932"; 
            journalName: "Yeast"; date: "2012-12"; volume: "29"; issue: "12"; 
            pages: "519-30"
COMMENT     Sequence Label: pNC1137
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..760)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(90..110)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     705..733
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaaatcataa atcataagaa attcgcttat ttagaagtgg cgcgccctat ttgtatagtt
       61 catccatgcc atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtggtctc
      121 tcttttcgtt gggatctttc gaaagggcag attgtgtgga caggtaatgg ttgtctggta
      181 aaaggacagg gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgc
      241 ttccatcttc aatgttgtgt ctaattttga agttagcttt gattccattc ttttgtttgt
      301 ctgccatgat gtatacattg tgtgagttat agttgtattc caatttgtgc ccaagaatgt
      361 ttccatcttc tttaaaatca atacctttta actcgattct attaacaagg gtatcacctt
      421 caaacttgac ttcagcacgt gtcttgtagt tcccgtcatc tttgaaaaat atagttcttt
      481 cctgtacata accttcgggc atggcactct tgaaaaagtc atgctgtttc atgtgatctg
      541 ggtatcttga aaagcattga acaccataag tgagagtagt gacaagtgtt ggccatggaa
      601 caggtagttt tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac
      661 cttcaccctc tccactgaca gaaaatttgt gcccattaac atcaccatct aattcaacaa
      721 gaattgggac aactccagtg aaaagttctt ctcctttact cgtattgggc gccagggtgg
      781 tttttctttt caccagtgag acgggcaaca gccaagctcc ggat
//
