LOCUS       sequence-59338-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Lentini R, Forlin M, Martini L, Del Bianco C, Spencer AC, Torino D, 
            Mansy SS
  TITLE     Fluorescent Proteins and in Vitro Genetic Organization for Cell-Free
            Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2013 Mar 15.
  PUBMED    23654270
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Mar 15."
COMMENT     Sequence Label: pET21b-RL015A
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..745
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     RBS             764..786
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 actttaagaa ggagatatac atatggctag catgcgtaaa ggcgaagagc tgttcactgg
       61 tgtcgtccct attctggtgg aactggatgg tgatgtcaac ggtcataagt tttccgtgcg
      121 tggcgagggt gaaggtgacg caactaatgg taaactgacg ctgaagttca tctgtactac
      181 tggtaaactg ccggtacctt ggccgactct ggtaacgacg ctgacttatg gtgttcagtg
      241 ctttgctcgt tatccggacc atatgaagca gcatgacttc ttcaagtccg ccatgccgga
      301 aggctatgtg caggaacgca cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc
      361 ggaagtgaaa tttgaaggcg ataccctggt aaaccgcatt gagctgaaag gcattgactt
      421 taaagaagac ggcaatatcc tgggccataa gctggaatac aattttaaca gccacaatgt
      481 ttacatcacc gccgataaac aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa
      541 cgtggaggat ggcagcgtgc agctggctga tcactaccag caaaacactc caatcggtga
      601 tggtcctgtt ctgctgccag acaatcacta tctgagcacg caaagcgttc tgtctaaaga
      661 tccgaacgag aaacgcgatc atatggttct gctggagttc gtaaccgcag cgggcatcac
      721 gcatggtatg gatgaactgt acaaataagc ggatccgaat aattttgttt aactttaaga
      781 aggagatata catatggtga gtaaaggcga ggaggacaat atggcgatca tc
//
