LOCUS       sequence-59361-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Zhu Q, Pao GM, Huynh AM, Suh H, Tonnu N, Nederlof PM, Gage FH, Verma
            IM
  TITLE     BRCA1 tumour suppression occurs via heterochromatin-mediated 
            silencing.
  JOURNAL   Nature. 2011 Sep 7;477(7363):179-84. doi: 10.1038/nature10371.
  PUBMED    21901007
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-09-7- 7"; volume: "477"; issue: "7363"; pages:
            "179-84"
COMMENT     Sequence Label: p156RRL-EF1a-GFP-U3H1SatA
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    54..171
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      256..265
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(262..264,265..267,268..780)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(307..328)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(568..587)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgtggaggaa tttcgacatt taaatttaat taatctcgac ggtatcggtt aacttttaaa
       61 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
      121 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac
      181 gagactagcc tcgaggttta aactacggga tcttcgaagg cctaagctta cgcgcgcgtc
      241 ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
      301 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      361 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      421 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
      481 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      541 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      601 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      661 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      721 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
      781 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggccc
//
