LOCUS       sequence-59363-e         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Zhu Q, Pao GM, Huynh AM, Suh H, Tonnu N, Nederlof PM, Gage FH, Verma
            IM
  TITLE     BRCA1 tumour suppression occurs via heterochromatin-mediated 
            silencing.
  JOURNAL   Nature. 2011 Sep 7;477(7363):179-84. doi: 10.1038/nature10371.
  PUBMED    21901007
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-09-7- 7"; volume: "477"; issue: "7363"; pages:
            "179-84"
COMMENT     Sequence Label: pBOB-CAG-fHis-H2A-Ub
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..89
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             480..707
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             480..707
                     /codon_start=1
                     /gene="Homo sapiens UBC"
                     /product="human ubiquitin C"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 tgtctcatca ttttggcaaa gaattcgcta gtggatccac catggtgcat catcatcatc
       61 atcacgatta caaggatgac gacgataaga tgtctgggcg tggtaagcag ggtggcaagg
      121 cgcgcgccaa ggctaagtcg cgctcgtcgc gcgcggggct gcagttcccc gtgggccgcg
      181 tgcaccggtt gctccgcaag ggcaactatt cggagcgcgt gggcgccggc gccccggtct
      241 atctggccgc ggtgctcgag tacttgactg ccgagatcct ggagcttgcc ggcaacgcgg
      301 cgcgcgacaa caagaagacg cgcatcatcc cgcgccacct gcagctggcc atccgcaacg
      361 acgaggagct caacaagctg ctgggccgcg tgaccatcgc gcagggtggc gtcctgccca
      421 acatccaggc cgtgctgctg ccaaagaaaa ctgagagcca ccacaaggcg aagggcaaga
      481 tgcagatctt cgtgaagacc ctgactggta agaccatcac tctcgaagtg gagccgagtg
      541 acaccattga gaatgtcaag gcaaagatcc aagacaagga aggcatccct cctgaccagc
      601 agaggttgat ctttgctggg aaacagctgg aagatggacg caccctgtct gactacaaca
      661 tccagaaaga gtccaccctg cacctggtcc tccgtctcag aggtgggtaa gataacgtct
      721 cgagtttaaa cctggaaaa
//
