LOCUS       sequence-59385-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PHPT1A
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             91..111
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             543..560
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(613..631)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      627..674
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(798..817)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgca ccatcatcat
       61 catcattctt ctggtgtaga tctgggtacc gagaacctgt acttccaatc catggacctc
      121 gctctcattc ctgatgtgga catcgactcc gacggcgtct tcaagtatgt gctgatccga
      181 gtccactcgg ctccccgctc cggggctccg gctgcagaga gcaaggagat cgtgcgcggc
      241 tacaagtggg ctgagtacca tgcggacatc tacgacaaag tgtcgggcga catgcagaag
      301 caaggctgcg actgtgagtg tctgggcggc gggcgcatct cccaccagag tcaggacaag
      361 aagattcacg tgtacggcta ttccatggcc tatggtcctg cccagcacgc catttcaact
      421 gagaaaatca aagccaagta ccccgactac gaggtcacct gggctaacga cggctactga
      481 cagtaaaggt ggatacggat ccgaattcga gctccgtcga caagcttgcg gccgcactcg
      541 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
      601 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
      661 tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg
      721 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      781 cagcgcccta gcgcccgctc ctttcgcttt cttccctttc ctttctcgcc acgttcgccg
      841 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
      901 ggcacctcga ccccaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
      961 atagacggtt tt
//
