LOCUS       sequence-59387-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DDX3XA
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cctctagaaa taattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atggttgagg
      121 caacaggcaa caactgtcct ccacatattg aaagtttcag tgatgttgag atgggagaaa
      181 ttatcatggg aaacattgag cttactcgtt atactcgccc aactccagtg caaaagcatg
      241 ctattcctat tatcaaagag aaaagagact tgatggcttg tgcccaaaca gggtctggaa
      301 aaactgcagc atttctgttg cccatcttga gtcagattta ttcagatggt ccaggcgagg
      361 ctttgagggc catgaaggaa aatggaaggt atgggcgccg caaacaatac ccaatctcct
      421 tggtattagc accaacgaga gagttggcag tacagatcta cgaggaagcc agaaaatttt
      481 cataccgatc tagagttcgt ccttgcgtgg tttatggtgg tgccgatatt ggtcagcaga
      541 ttcgagactt ggaacgtgga tgccatttgt tagtagccac tccaggacgt ctagtggata
      601 tgatggaaag aggaaagatt ggattagact tttgcaaata cttggtgtta gatgaagctg
      661 atcggatgtt ggatatgggg tttgagcctc agattcgtag aatagtcgaa caagatacta
      721 tgcctccaaa gggtgtccgc cacactatga tgtttagtgc tacttttcct aaggaaatac
      781 agatgctggc tcgtgatttc ttagatgaat atatcttctt ggctgtagga agagttggct
      841 ctacctctga aaacatcaca cagaaagtag tttgggtgga agaatcagac aaacggtcat
      901 ttctgcttga cctcctaaat gcaacaggca aggattcact gaccttagtg tttgtggaga
      961 ccaaaa
//
