LOCUS       sequence-59388-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ALDRL6A
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             91..111
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgca ccatcatcat
       61 catcattctt ctggtgtaga tctgggtacc gagaacctgt acttccaatc catggaccgt
      121 gtcttcacca cctacaagct catgcacacg caccagacag tggacttcgt caggagcaag
      181 catgcccagt ttgggggctt ctcctacaag aaaatgacag tcatggaggc cgtggacctg
      241 ctggatgggc tggtggatga gtcggacccg gacgtagatt tccccaactc cttccatgcc
      301 ttccagacag cggagggcat ccggaaggcc cacccagaca aggactggtt ccacctcgtc
      361 gggctcctgc acgacctggg gaaggtcctg gccctgttcg gggagcccca gtgggctgtc
      421 gtcggcgaca ccttccccgt cggatgccgt ccgcaggcct ccgtggtttt ctgcgactcc
      481 accttccagg acaaccctga cctccaggat cctcgataca gcacagagct cgggatgtat
      541 cagccccact gtgggctcga cagggtcctc atgtcctggg gccatgatga gtacatgtac
      601 caggtgatga agtttaacaa gttctcactg ccccctgagg ctttctacat gatccggttc
      661 cactccttct acccctggca cacgggccgc gactaccagc agctgtgcag ccagcaggac
      721 ctggccatgc tgccctgggt gcgggagttc aacaagttcg acctctacac caagtgcccg
      781 gacctgccgg acgtggacaa gctgcggccc tactaccagg ggctcattga caagtactgc
      841 cctggcatcc tgagctggtg acagtaaagg tggatacgga tccgaattcg agctccgtcg
      901 acaagcttgc ggccgcactc gagcaccacc acacacatga a
//
