LOCUS       sequence-59391-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CPSF5A
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             91..111
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             792..809
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(862..880)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      876..923
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgca ccatcatcat
       61 catcattctt ctggtgtaga tctgggtacc gagaacctgt acttccaatc catgtacatc
      121 cagcagacga agcccctcac cctggagcgc accatcaacc tgtaccctct taccaattat
      181 acttttggta caaaagagcc cctctacgag aaggacagct ctgttgcagc cagatttcag
      241 cgcatgaggg aagaatttga taaaattgga atgaggagga ctgtagaagg ggttctgatt
      301 gtacatgagc accggctacc ccatgtgtta ctgctgcagc tgggaacaac tttcttcaaa
      361 ctacctggtg gtgaacttaa cccaggagaa gatgaagttg aaggactaaa acgcttaatg
      421 acagagatac tgggtcgtca ggatggagtt ttgcaagact gggtcattga cgattgcatt
      481 ggtaactggt ggagaccaaa ttttgaacct cctcagtatc catatattcc tgcacatatt
      541 acaaagccta aggaacataa gaagttgttt ctggttcagc ttcaagaaaa agccttgttt
      601 gcagtcccta aaaattacaa gctggtagct gcaccattgt ttgaattgta tgacaatgca
      661 ccaggatatg gacccatcat ttctagtctc cctcagctgt tgagcaggtt caattttatt
      721 tacaactgac agtaaaggtg gatacggatc cgaattcgag ctccgtcgac aagcttgcgg
      781 ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg
      841 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
      901 aacgggtctt gaggggtttt ttgctgaaga gactat
//
