LOCUS       sequence-59393-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ASSA
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgtcc
      121 agcaaaggct ccgtggttct ggcctacagt ggcggcctgg acacctcgtg catcctcgtg
      181 tggctgaagg aacaaggcta tgacgtcatt gcctatctgg ccaacattgg ccagaaggaa
      241 gacttcgagg aagccaggaa gaaggcactg aagcttgggg ccaaaaaggt gttcattgag
      301 gatgtcagca gggagtttgt ggaggagttc atctggccgg ccatccagtc cagcgcactg
      361 tatgaggacc gctacctcct gggcacctct cttgccaggc cctgcatcgc ccgcaaacaa
      421 gtggaaatcg cccagcggga gggggccaag tatgtgtccc acggcgccac aggaaagggg
      481 aacgatcagg tccggtttga gctcagctgc tactcactgg ccccccagat aaaggtcatt
      541 gctccctgga ggatgcctga attctacaac cggttcaagg gccgcaatga cctgatggag
      601 tacgcaaagc aacacgggat tcccatcccg gtcactccca agaacccgtg gagcatggat
      661 gagaacctca tgcacatcag ctacgaggct ggaatcctgg agaaccccaa gaaccaagcg
      721 cctccaggtc tctacacgaa gacccaggac ccagccaaag cccccaacac ccctgacatt
      781 ctcgagatcg agttcaaaaa aggggtccct gtgaaggtga ccaacgtcaa ggatggcacc
      841 acccaccaga cctccttgga gctcttcatg tacctgaacg aagtcgcggg caagcatggc
      901 gtgg
//
