LOCUS       sequence-59394-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PPP2R5CA
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatggtg
      121 gtggatgcgg ccaactccaa tgggcctttc cagcccgtgg tccttctcca tattcgagat
      181 gttcctcctg ctgatcaaga gaagcttttt atccagaagt tacgtcagtg ttgcgtcctc
      241 tttgactttg tttctgatcc actaagtgac ctaaagtgga aggaagtaaa acgagctgct
      301 ttaagtgaaa tggtagaata tatcacccat aatcggaatg tgatcacaga gcctatttac
      361 ccagaagtag tccatatgtt tgcagttaac atgtttcgaa cattaccacc ttcctccaat
      421 cctacgggag cggaatttga cccggaggaa gatgaaccaa cgttagaagc agcctggcct
      481 catctacagc ttgtttatga atttttctta agatttttag agtctccaga tttccaacct
      541 aatatagcga agaaatatat tgatcagaag tttgtattgc agcttttaga gctctttgac
      601 agtgaagatc ctcgggagag agattttctt aaaaccaccc ttcacagaat ctatgggaaa
      661 ttcctaggct tgagagctta catcagaaaa cagataaata atatatttta taggtttatt
      721 tatgaaacag agcatcataa tggcatagca gagttactgg aaatattggg aagtataatt
      781 aatggatttg ccttaccact aaaagaagag cacaagattt tcttattgaa ggtgttacta
      841 cctttgcaca aagtgaaatc tctgagtgtc taccatcccc agctggcata ctgtgtagtg
      901 cagtttttag aaaaggacag caccctcacg gaaccagtgg tg
//
