LOCUS       sequence-59395-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PRTFDC1A
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             855..872
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(925..943)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatggcc
      121 gggagcagcg aggaggcgcc agactacggg cgaggcgtcg tgattatgga tgattggcca
      181 gggtatgact tgaatttatt cacgtaccca cagcactatt atggagactt ggagtatgtc
      241 ctcatccctc atggtatcat tgtggacaga attgagcggc tggccaagga tattatgaaa
      301 gacataggat atagtgacat catggtcctg tgtgtgctta aaggaggtta caaattctgt
      361 gctgatctcg tagaacacct taagaacatc agccgaaatt cagatcgatt tgtctcaatg
      421 aaggttgatt tcatcagact aaaaagttac aggaatgacc agtccatggg tgagatgcag
      481 ataatcggag gcgatgatct ttcaacgctg gctggaaaga atgttctcat tgttgaggat
      541 gttgtcggaa ctgggaggac catgaaagca ctactcagca atatagagaa atacaagccc
      601 aacatgatta aggtagccag tttgttggtg aagagaacat ccagaagtga cggctttaga
      661 cctgactatg ctggatttga gattccaaac ttatttgtgg tgggatatgc cttagattac
      721 aatgaatact tcagagatct gaatcacata tgcgtcatca atgagcacgg taaagaaaaa
      781 tatcgagtct gacagtaaag gtggatacgg atccgaattc gagctccgtc gacaagcttg
      841 cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
      901 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc tggc
//
