LOCUS       sequence-59401-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FLT1A
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgcc
      121 ggatgaagtg ccactggacg agcagtgcga acgtctgcct tatgatgcga gcaaatggga
      181 gtttgcccgc gaacgtctga aactgggcaa atctctgggt cgcggggcat tcggaaaagt
      241 tgtccaagct agtgcgtttg gcattaaaaa atcaccgacc tgtcgtacgg tagccgtgaa
      301 aatgctgaaa gaaggtgcaa ctgcttccga gtacaaagcg ctgatgacag aactgaaaat
      361 cctgacccat attgggcacc atctgaatgt tgtcaacctg ctgggagcct gcacgaaaca
      421 gggcggtcca ctgatggtaa tcgtggagta ttgtaaatac ggcaatctgt cgaactatct
      481 gaaaagcaaa cgcgacctgt tctttctgaa taaagatgca gctctgcaca tggaacctaa
      541 aaaagaaaaa atggagccgg gtctggaaca agggaaaaaa ccgcgtctgg actctgttac
      601 tagttcagag tccttcgcgt cgtcaggttt ccaggaagat aaatctctgt ctgacgtgga
      661 agaggaagag gactcagatg gcttctacaa agaaccaatt acaatggagg acctgatctc
      721 ctattcgttt caggtggccc gcggtatgga gttcctgagc tctcgtaaat gcattcatcg
      781 cgatctggca gctcgtaaca tcctgctgag tgaaaataac gtagttaaaa tttgtgactt
      841 tggcctggcg cgcgatatct acaaaaatcc tgattatgtg cgtaaaggtg acacccgcct
      901 gccgctgaaa tggatggccc cggaaagtat ctttgataaa atctatt
//
