LOCUS       sequence-59404-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TNKS2A
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             835..852
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(905..923)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgtt
      121 gaacacctct ggtagtggaa caattcttat agatctgtct cctgatgata aagagtttca
      181 gtctgtggag gaagagatgc aaagtacagt tcgagagcac agagatggag gtcatgcagg
      241 tggaatcttc aacagataca atattctcaa gattcagaag gtttgtaaca agaaactatg
      301 ggaaagatac actcaccgga gaaaagaagt ttctgaagaa aaccacaacc atgccaatga
      361 acgaatgcta tttcatgggt ctccttttgt gaatgcaatt atccacaaag gctttgatga
      421 aaggcatgcg tacataggtg gtatgtttgg agctggcatt tattttgctg aaaactcttc
      481 caaaagcaat caatatgtat atggaattgg aggaggtact gggtgtccag ttcacaaaga
      541 cagatcttgt tacatttgcc acaggcagct gctcttttgc cgggtaacct tgggaaagtc
      601 tttcctgcag ttcagtgcaa tgaaaatggc acattctcct ccaggtcatc actcagtcac
      661 tggtaggccc agtgtaaatg gcctagcatt agctgaatat gttatttaca gaggagaaca
      721 ggcttatcct gagtatttaa ttacttacca gattatgagg cctgaaggtt gacagtaaag
      781 gtggatacgg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac
      841 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
      901 gccaccgctg agcaataact agc
//
