LOCUS       sequence-59406-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PARP14A
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             757..774
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(827..845)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      841..888
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatggg
      121 gcagaagtgc ttctctcgga cagtcttggc ccctggcgtt gtgctgattg tgcagcaggg
      181 tgacttggca cggcttcctg tcgatgtggt ggtgaatgca tctaatgagg accttaagca
      241 ttatggtggc ctggccgctg cgctctcaaa agcagctggc cctgagctcc aggccgactg
      301 tgaccagata gtgaagagag agggcagact cctaccgggc aatgccacca tctccaaggc
      361 aggaaagctg ccctaccacc acgtgatcca tgcagtgggg ccccgctgga gcggatatga
      421 ggccccgagg tgtgtgtacc tattaaggag agctgtgcaa ctcagtctct gtctagccga
      481 aaaatacaag taccgatcca tagccatccc agctattagt tctggagtct ttggctttcc
      541 cttaggccga tgcgtggaga ccattgtttc tgccatcaag gaaaacttcc aattcaagaa
      601 ggatggacac tgcttgaaag aaatctacct tgtggatgta tctgagaaga ctgttgaggc
      661 ctttgcagaa gctgtgaaaa ctgtatttaa atgacagtaa aggtggatac ggatccgaat
      721 tcgagctccg tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactgagat
      781 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
      841 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
      901 actatatccg gattggcga
//
