LOCUS       sequence-59409-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PARP14A
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             778..795
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(849..867)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatggg
      121 gaaaacatca tgggaaaaag gaagcctggt gtccccggga ggcctgcaga tgctgttggt
      181 gaaagagggt gtgcagaatg ctaagaccga tgttgttgtc aactccgttc ccttggatct
      241 cgtgcttagt agagggcctc tttctaagtc cctcttggaa aaagctggac cagagctcca
      301 ggaggaattg gacacagttg gacaaggggt ggctgtcagc atgggcacag tgctcaaaac
      361 cagcagctgg aatctggact gtcgctatgt gcttcacgtg gtagctccgg agtggagaaa
      421 tggtagcaca tcttcactca agataatgga agacataatc agagaatgta tggagatcac
      481 tgagagcttg tccttaaaat caattgcatt tccagcaata ggaacaggaa acttgggatt
      541 tcctaaaaac atattcgctg aattaatcat ttcagaggtg ttcaaattta gtagcaagaa
      601 tcagctgaaa actttacaag aggttcactt tctgctgcac ccgagtgatc atgaaaatat
      661 tcaggcattt tcagatgaat ttgccagaag ggctaatgga aatctcgtca gttgacagta
      721 aaggtggata cggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac
      781 caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgaagttggc
      841 tgctgccacc gctgagcaat aactagcata accccttgg
//
