LOCUS       sequence-59413-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PARP14A
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             757..774
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(827..845)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      841..888
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgga
      121 tatgaaacaa cagaactttt gcgttgtcga actgctgcca tccgaccctg agtataatac
      181 agtagcttcg aaattcaacc aaacctgtag ccattttcgt attgaaaaaa tcgaacgcat
      241 tcagaatccg gatctgtgga actcttacca ggcgaaaaaa aaaacgatgg atgccaaaaa
      301 tggtcaaacc atgaacgaga aacagctgtt ccacgggact gacgcaggaa gtgtgccaca
      361 tgttaatcgt aacggcttta atcgctcata tgctggtaaa aacgcggtcg cctacgggaa
      421 aggaacatat ttcgcagtga atgctaacta ctccgcgaat gatacgtatt cgcgtcctga
      481 cgccaacggc cgcaaacacg tatattacgt tcgtgtgctg accggtatct atactcatgg
      541 caatcattca ctgattgtcc cgccgtctaa aaacccacaa aatcctacag atctgtacga
      601 caccgttacg gataacgtac atcacccgag tctgtttgtg gcattctatg attaccaggc
      661 ttatccggaa tacctgatca cctttcgcaa atgacagtaa aggtggatac ggatccgaat
      721 tcgagctccg tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactgagat
      781 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
      841 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
      901 actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaa
//
