LOCUS       sequence-59415-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NUDT14A
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..115
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             769..786
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(839..857)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      853..900
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgcaccatca
       61 tcatcatcat tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgaa
      121 tggtgcccag aagtcctggg acttcatgaa gacgcatgac agcgtgaccg ttctcttatt
      181 caactcttct cggaggagcc tggtgttggt gaagcagttc cggccagctg tgtatgcggg
      241 tgaggtggag cgccgcttcc cagggtccct agcagctgta gaccaggacg ggcctcggga
      301 gctacagcca gccctgcccg gctcagcggg ggtgacagtt gagctgtgtg ccggcctcgt
      361 ggaccagcct gggctctcgc tggaggaagt ggcttgcaag gaggcttggg aggagtgtgg
      421 ctaccacttg gccccctctg atctgcgccg ggtcgccaca tactggtctg gagtgggact
      481 gactggctcc agacagacca tgttctacac agaggtgaca gatgcccagc gtagcggtcc
      541 aggtgggggc ctggtggagg agggtgagct cattgaggtg gtgcacctgc ccctggaagg
      601 cgcccaggcc tttgcagacg acccggacat ccccaagacc ctcggcgtca tctttggtgt
      661 ctcatggttc ctcagccagg tggcccccaa cctggatctc cagtgacagt aaaggtggat
      721 acggatccga attcgagctc cgtcgacaag cttgcggccg cactcgagca ccaccaccac
      781 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      841 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      901 ctgaaaggag gaactatatc cggattggcg aatgggac
//
