LOCUS       sequence-59420-e         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UCK1A
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             849..866
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(919..937)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgcgg
      121 cccttcctga taggggtgag cggcggcact gccagcggga agtcgaccgt gtgtgagaag
      181 atcatggagt tgctgggaca gaacgaggtg gaacagcggc agcggaaggt ggtcatcctg
      241 agccaggaca ggttctacaa ggtcctgacg gcagagcaga aggccaaggc cttgaaagga
      301 cagtacaatt ttgaccatcc agatgccttt gataatgatt tgatgcacag gactctgaag
      361 aacatcgtgg agggcaaaac ggtggaggtg ccgacctatg attttgtgac acactcaagg
      421 ttaccagaga ccacggtggt ctaccctgcg gacgtggttc tgtttgaggg catcttggtg
      481 ttctacagcc aggagatccg ggacatgttc cacctgcgcc tcttcgtgga caccgactcc
      541 gacgtcaggc tgtctcgaag agttctccgg gacgtgcgcc gagggaggga cctggagcag
      601 attctgacgc agtacaccac cttcgtgaag ccggccttcg aggagttctg cctgccgaca
      661 aagaagtatg ccgatgtgat catcccgcga ggagtggaca atatggttgc catcaacctg
      721 atcgtgcagc acatccagga cattctgaat ggtgacatct gcaaatggca ccgaggaggg
      781 tcctgacagt aaaggtggat acggatccga attcgagctc cgtcgacaag cttgcggccg
      841 cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag
      901 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      961 gggtcttgag gttt
//
