LOCUS       sequence-59421-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UMPSA
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             951..968
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatggaa
      121 ctcagcttcg gtgcacgtgc agagctgccc aggatccacc cagttgcatc gaagcttctc
      181 aggcttatgc aaaagaagga gaccaatctg tgtctatctg ctgatgtttc actggccaga
      241 gagctgttgc agctagcaga tgctttagga cctagtatct gcatgctgaa gactcatgta
      301 gatattttga atgattttac tctggatgtg atgaaggagt tgataactct ggcaaaatgc
      361 catgagttct tgatatttga agaccggaag tttgcagata taggaaacac agtgaaaaag
      421 cagtatgaag gaggtatctt taaaatagct tcctgggcag atctagtaaa tgctcacgtg
      481 gtgccaggct caggagttgt gaaaggcctg caagaagtgg gcctgccttt gcatcggggg
      541 tgcctcctta ttgcggaaat gagctccacc ggctccctgg ccactgggga ctacactaga
      601 gcagcggtta gaatggctga ggagcactct gaatttgttg ttggttttat ttctggctcc
      661 cgagtaagca tgaaaccaga atttcttcac ttgactccag gagttcagtt ggaagcagga
      721 ggagataatc ttggccaaca gtacaatagc ccacaagaag ttattggcaa acgaggttcc
      781 gatatcatca ttgtaggtcg tggcataatc tcagcagctg atcgtctgga agcagcagag
      841 atgtacagaa aagctgcttg ggaagcgtat ttgagtagac ttggttgaca gtaaaggtgg
      901 atacggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc
      961 accaccac
//
