LOCUS       sequence-59423-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PRPSAP2A
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             692..703
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgaac
      121 ataaccaaag gtggtctggt gttgttttca gcaaactcga attcatcatg tatggagcta
      181 tcaaagaaaa ttgcagagcg gctaggggtg gagatgggca aagtgcaggt ttaccaggaa
      241 cctaacagag aaacaagagt acaaattcaa gagtctgtga ggggaaaaga tgttttcatc
      301 atccaaactg tttcgaagga cgtgaacacc accatcatgg agctcctgat catggtgtat
      361 gcatgtaaga cctcttgtgc caagagcatc attggcgtga taccctactt tccttacagc
      421 aagcagtgca agatgagaaa aagaggctcc attgtctcta aattgctggc ttccatgatg
      481 tgcaaagctg gtctaactca tcttattact atggatttac accagaagga aattcagggc
      541 ttcttcaata ttcctgttga caatttaaga gcatctccct tcttattaca gtatattcaa
      601 gaagagatcc cagattacag gaatgcagta atcgtggcca agtctccagc ctcggcgaag
      661 agggcacagt cttttgctga gcgcctgcgc ctgggaattg cagtgattca tggagaggcg
      721 caggatgccg agtcggactt ggtggatgga cggcattccc cacccatggt cagaagtgtg
      781 gctgccatcc accccagcct ggagatcccc atgctgattc ctaaagaaaa gcccccaatc
      841 acggttgtgg gtgatgttgg aggaaggatt gccatcatcg tggatgacat cattgatgat
      901 gttgacagct ttcttgctgc agcagagacc ctgaaggaaa gaggtgcata taagatcttt
      961 gtgatggcaa ctcatg
//
