LOCUS       sequence-59424-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BIRC3A
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             465..482
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(535..553)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      549..596
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(720..739)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     930..951
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgaga
      121 tacacagttt ctaatctgag catgcagaca catgcagccc gctttaaaac attctttaac
      181 tggccctcta gtgttctagt taatcctgag cagcttgcaa gtgcgggttt ttattatgtg
      241 ggtaacagtg atgatgtcaa atgcttttgc tgtgatggtg gactcaggtg ttgggaatct
      301 ggagatgatc catgggttca acatgccaag tggtttccaa ggtgtgagta cttgataaga
      361 attaaaggac aggagttcat ccgtcaagtt caagccagtt gacagtaaag gtggatacgg
      421 atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc
      481 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      541 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      601 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
      661 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      721 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      781 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
      841 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
      901 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
      961 caacccta
//
