LOCUS       sequence-59425-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DDX41A
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             687..704
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(757..775)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      771..818
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgggg
      121 gctgccagcc tggatgtcat ccaggaggta gaatatgtga aggaggaggc caagatggtg
      181 tacctgctcg agtgcctgca gaagacaccc ccgcctgtac tcatctttgc agagaagaag
      241 gcagacgtgg acgccatcca cgagtacctg ctgctcaagg gggttgaggc cgtagccatc
      301 catgggggca aagaccagga ggaacggact aaggccatcg aggcattccg ggagggcaag
      361 aaggatgtcc tagtagccac agacgttgcc tccaagggcc tggacttccc tgccatccag
      421 cacgtcatca attatgacat gccagaggag attgagaact atgtacaccg gattggccgc
      481 accgggtgct cgggaaacac aggcatcgcc actaccttca tcaacaaagc gtgtgatgag
      541 tcagtgctga tggacctcaa agcgctgctg ctagaagcca agcagaaggt gccgcccgtg
      601 ctgcaggtgc tgcattgcgg gtgacagtaa aggtggatac ggatccgaat tcgagctccg
      661 tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta
      721 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      781 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      841 gattggcgaa tgggacgcgc cctgtagcgg cgcatta
//
