LOCUS       sequence-59426-e         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PARP3A
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             93..113
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg caccatcatc
       61 atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa tccatgaagc
      121 gggtgcagcc ctgctccctg gacccagcca cgcagaagct catcactaac atcttcagca
      181 aggagatgtt caagaacacc atggccctca tggacctgga tgtgaagaag atgcccctgg
      241 gaaagctgag caagcaacag attgcacggg gtttcgaggc cttggaggcg ctggaggagg
      301 ccctgaaagg ccccacggat ggtggccaaa gcctggagga gctgtcctca cacttttaca
      361 ccgtcatccc gcacaacttc ggccacagcc agcccccgcc catcaattcc cctgagcttc
      421 tgcaggccaa gaaggacatg ctgctggtgc tggcggacat cgagctggcc caggccctgc
      481 aggcagtctc tgagcaggag aagacggtgg aggaggtgcc acaccccctg gaccgagact
      541 accagcttct caagtgccag ctgcagctgc tagactctgg agcacctgag tacaaggtga
      601 tacagaccta cttagaacag actggcagca accacaggtg ccctacactt caacacatct
      661 ggaaagtaaa ccaagaaggg gaggaagaca gattccaggc ccactccaaa ctgggtaatc
      721 ggaagctgct gtggcatggc accaacatgg ccgtggtggc cgccatcctc actagtgggc
      781 tccgcatcat gccacattct ggtgggcgtg ttggcaaggg catctacttt gcctcagaga
      841 acagcaagtc agctggatat gttattggca tgaagtgtgg ggcccaccat gtcggctaca
      901 tgttcctggg tgaggtggcc ctgggcagag agcaccatat caacacggac aaccccagct
      961 tgaagagccc acct
//
