LOCUS       sequence-59428-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GUADA
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgtgt
      121 gccgctcaga tgccgcccct ggcgcacatc ttccgaggga cgttcgtcca ctccacctgg
      181 acctgcccca tggaggtgct gcgggatcac ctcctcggcg tgagcgacag cggcaaaata
      241 gtgtttttag aagaagcatc tcaacaggaa aaactggcca aagaatggtg cttcaagccg
      301 tgtgaaataa gagaactgag ccaccatgag ttcttcatgc ctgggctggt tgatacacac
      361 atccatgcct ctcagtattc ctttgctgga agtagcatag acctgccact cttggagtgg
      421 ctgaccaagt acacatttcc tgcagaacac agattccaga acatcgactt tgcagaagaa
      481 gtatatacca gagttgtcag gagaacacta aagaatggaa caaccacagc ttgttacttt
      541 gcaacaattc acactgactc atctctgctc cttgccgaca ttacagataa atttggacag
      601 cgggcatttg tgggcaaagt ttgcatggat ttgaatgaca cttttccaga atacaaggag
      661 accactgagg aatcgatcaa ggaaactgag agatttgtgt cagaaatgct ccaaaagaac
      721 tattctagag tgaagcccat agtgacacca cgtttttccc tctcctgctc tgagactttg
      781 atgggtgaac tgggcaacat tgctaaaacc cgtgatttgc acattcagag ccatataagt
      841 gaaaatcgtg atgaagttga agctgtgaaa aacttatacc ccagttataa aaactacaca
      901 tctgtgtatg ataaaaacaa tct
//
