LOCUS       sequence-59429-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PARP12A
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             771..788
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(841..859)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      855..902
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatggac
      121 tcctctgccc tgccagaccc aggctttcag aagatcaccc ttagttcttc ctcggaagag
      181 tatcagaagg tctggaacct ctttaaccgc acgctgcctt tctactttgt tcagaagatt
      241 gagcgagtac agaacctggc cctctgggaa gtctaccagt ggcaaaaagg acagatgcag
      301 aagcagaatg gagggaaggc cgtggacgag cggcagctgt tccacggcac cagcgccatt
      361 tttgtggacg ccatctgcca gcagaacttt gactggcggg tctgtggtgt tcatggcact
      421 tcctacggca aggggagcta ctttgcccga gatgctgcat attcccacca ctacagcaaa
      481 tccgacacgc agacccacac gatgttcctg gcccgggtgc tggtgggcga gttcgtcagg
      541 ggcaatgcgt cctttgtccg tccgccggcc aaggagggct ggagcaacgc cttctatgat
      601 agctgcgtga acagtgtgtc cgacccctcc atctttgtga tctttgagaa acaccaggtc
      661 tacccagagt atgtcatcca gtacaccacc tcctccaagc cctcgtgaca gtaaaggtgg
      721 atacggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc
      781 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      841 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      901 tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt
      961 aagcgcggcg gggtgtg
//
