LOCUS       sequence-59430-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: STARD13A
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             825..842
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(895..913)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      909..956
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatggag
      121 gagagtgggg caactttcca cacttacctg aaccatctca tccagggcct ccagaaagaa
      181 gccaaggaga agttcaaagg atgggtcacg tgctccagca cggacaatac agatcttgct
      241 ttcaaaaagg tgggcgacgg gaacccgctg aagctgtgga aggcttctgt ggaggtggaa
      301 gcacccccct cagtggtcct gaaccgcgtg ctgagagagc gccacctgtg ggacgaggac
      361 tttgtgcagt ggaaggttgt ggaaactcta gacaggcaaa cagagatcta ccagtatgtg
      421 ctgaacagca tggctcccca tccttccaga gactttgtgg ttctcaggac ctggaaaact
      481 gatttgccca aaggaatgtg taccctggtg tccctctccg tggagcatga ggaagcccag
      541 ctcctgggtg gtgtgcgagc agtggtgatg gactcgcagt acttgataga accgtgtggc
      601 tctggcaagt caagactgac tcacatctgc aggatagacc tgaaaggtca ctccccagaa
      661 tggtacagca aaggctttgg acatctgtgt gcagcagaag ttgccaggat tagaaactct
      721 ttccagcccc tcattgctga gggcccagaa actaaaatct gacagtaaag gtggatacgg
      781 atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc
      841 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      901 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      961 aa
//
