LOCUS       sequence-59431-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ADSS2A
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgccc
      121 agggcgcggc ccggaggaaa ccgggtgacg gtggtgctcg gtgcgcagtg gggcgacgaa
      181 ggcaaaggga aggtggtgga cctgctggcg caggacgccg acatcgtgtg ccgctgccag
      241 ggaggaaata atgctggcca tacagttgtt gtggattctg tggaatatga ttttcatctc
      301 ttacccagtg gaataattaa tccaaatgtc actgcattca ttggaaatgg tgtggtaatt
      361 catctacctg gattgtttga agaagcagag aaaaatgttc aaaaaggaaa aggactagaa
      421 ggctgggaaa aaaggcttat tatatctgac agagctcata ttgtatttga ttttcatcaa
      481 gcagctgatg gtatccagga acaacagaga caagaacaag caggaaaaaa tttgggtaca
      541 acaaaaaagg gcattggccc agtttattcg tccaaagctg ctcggagtgg actcaggatg
      601 tgcgaccttg tttctgactt tgatggcttc tctgagaggt ttaaagttct agctaaccaa
      661 tacaaatcta tataccccac tttggaaata gacattgaag gtgaattaca aaaactcaag
      721 ggttatatgg aaaagattaa accaatggtg agagatggag tttattttct atatgaggcc
      781 ctacatggac caccaaagaa aatcttggta gaaggtgcaa atgcagcact attagatatt
      841 gattttggga cttacccttt tgtaacctct tcaaattgta ctgttggagg tgtttgtact
      901 ggtttgggta tgccacctca aaatgttgga gaa
//
