LOCUS       sequence-59432-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CSADA
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatggct
      121 gactcagaag cactcccctc ccttgctggg gacccagtgg ctgtggaagc cttgctccgg
      181 gccgtgtttg gggttgttgt ggatgaggcc attcagaaag gaaccagtgt ctcccagaag
      241 gtctgtgagt ggaaggagcc tgaggagctg aagcagctgc tggatttgga gctgcggagc
      301 cagggcgagt cacagaagca gatcctggag cggtgtcggg ctgtgattcg ctacagtgtc
      361 aagactggtc accctcggtt cttcaaccag ctcttctctg ggttggatcc ccatgctctg
      421 gccgggcgca ttatcactga gagcctcaac accagccagt acacatatga aatcgccccc
      481 gtgtttgtgc tcatggaaga ggaggtgctg aggaaactgc gggccctggt gggctggagc
      541 tctggggacg gaatcttctg ccctggtggc tccatctcca acatgtatgc tgtaaatctg
      601 gcccgctatc agcgctaccc ggattgcaag cagaggggcc tccgcacact gccgcccctg
      661 gccctattca catcgaagga gtgtcactac tccatccaga agggagctgc gtttctggga
      721 cttggcaccg acagtgtccg agtggtcaag gctgatgaga gagggaaaat ggtccccgag
      781 gatctggaga ggcagattgg tatggccgag gctgagggtg ctgtgccgtt cctggtcagt
      841 gccacctctg gcaccactgt gctaggggcc tttgaccccc tggaggcaat tgctgatgtg
      901 tgccagcgtc atgggctatg gctgcatgtg gatgctgcct ggggtgggag cgtcctgctg
      961 tcacagacac acaggcat
//
