LOCUS       sequence-59433-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GARTA
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatggca
      121 gcccgagtac ttataattgg cagtggagga agggaacata cgctggcctg gaaacttgca
      181 cagtctcatc atgtcaaaca agtgttggtt gccccaggaa acgcaggcac tgcctgctct
      241 gaaaagattt caaataccgc catctcaatc agtgaccaca ctgcccttgc tcaattctgc
      301 aaagagaaga aaattgaatt tgtagttgtt ggaccagaag cacctctggc tgctgggatt
      361 gttgggaacc tgaggtctgc aggagtgcaa tgctttggcc caacagcaga agcggctcag
      421 ttagagtcca gcaaaaggtt tgccaaagag tttatggaca gacatggaat cccaaccgca
      481 caatggaagg ctttcaccaa acctgaagaa gcctgcagct tcattttgag tgcagacttc
      541 cctgctttgg ttgtgaaggc cagtggtctt gcagctggaa aaggggtgat tgttgcaaag
      601 agcaaagaag aggcctgcaa agctgtacaa gagatcatgc aggagaaagc ctttggggca
      661 gctggagaaa caattgtcat tgaagaactt cttgacggag aagaggtgtc gtgtctgtgt
      721 ttcactgatg gcaagactgt ggcccccatg cccccagcac aggaccataa gcgattactg
      781 gagggagatg gtggccctaa cacaggggga atgggagcct attgtccagc ccctcaggtt
      841 tctaatgatc tattactaaa aattaaagat actgttcttc agaggacagt ggatggcatg
      901 cagcaagagg gtactccata tac
//
