LOCUS       sequence-59434-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ACOT7A
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             693..710
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(763..781)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      777..824
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgggg
      121 gacatcgtcc agccagtcct caacccagag ccgaacactg tcagctacag ccagtccagc
      181 ttgatccacc tggtggggcc ttcagactgc accctgcacg gctttgtgca cggaggtgtg
      241 accatgaagc tcatggatga ggtcgccggg atcgtggctg cacgccactg caagaccaac
      301 atcgtcacag cttccgtgga cgccattaat tttcatgaca agatcagaaa aggctgcgtc
      361 atcaccatct cgggacgcat gaccttcacg agcaataagt ccatggagat cgaggtgttg
      421 gtggacgccg accctgttgt ggacagctct cagaagcgct accgggccgc cagtgccttc
      481 ttcacctacg tgtcgctgag ccaggaaggc aggtcgctgc ctgtgcccca gctggtgccc
      541 gagaccgagg acgagaagaa gcgctttgag gaaggcaaag ggcggtacct gcagatgaag
      601 gcgaagcgac agggccacgc ggagccttga cagtaaaggt ggatacggat ccgaattcga
      661 gctccgtcga caagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg
      721 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
      781 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
      841 tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
      901 ggttacgcgc agcgtgaccg ctacacttgg cag
//
